Assessment of genome stability of pfleFB hybrid generations through molecular DNA markers

Using PEG-mediated protoplast fusion, a total of nine pfle somatic hybrids were developed between Pleurotus florida and Lentinula edodes. Only six could fruit bodies the second generation was made each through tissue culture isolate from their bodies. The first denoted as pfle1p, pfle1q, pfle1v, 1s, pfle1o pfle1pFB, pfle1qFB, pfle1vFB, 1sFB, pfle1oFB. genetic closeness, stability variance is analyzed compared to parental strains using RAPD molecular markers. Amplified PCR bands scored SPSS software version 19. Polymorphisms found calculating 104 94.23% where highest polymorphism observed in RAPD-02, RAPD- 03, RAPD-08 SS-11. Maximum (16) generated by SRS-06 minimum SRS-05, ranged 330 bp – 3000 450 2000 bp, respectively. Jaccards proximity matrix data followed dendogram including two generations with parents, single linkage. 8.00-60.00. Two P. L. edodes be most distantly related among all, 1pFB, 1rFB very close distance another hybrid lines, 1vFB, 1qFB also showed same. It that nearest neighbor little genetically, but lines are mostly similar generation.